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Becton Dickinson
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Biozol Diagnostica Vertrieb GmbH
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Image Search Results
Journal: Clinical and Experimental Immunology
Article Title: Immunomodulatory glc/man‐directed Dolichos lablab lectin (DLL) evokes anti‐tumour response in vivo by counteracting angiogenic gene expressions
doi: 10.1111/cei.12959
Figure Lengend Snippet: Translational down‐regulation of tumoral angiogenic gene expression by Dolichos lablab lectin (DLL). Immunoblots, gelatin zymography and immunohistochemistry (IHC) were carried out using in‐vivo tumour cells of both Dalton's ascites lymphoma (DLA) and Dalton's lymphoma solid tumour (DLS) with or without DLL treatment in two different concentrations. (a,b) Immunoblots showing altered translational expression of proangiogenic genes such as nuclear factor kappa B (NF‐κB), inhibitory kappa B (I‐κB), hypoxia inducible factor 1α (HIF‐1α), vascular endothelial growth factor (VEGF)‐A, matrix metalloproteinase (MMP)‐2 and 9 in ascitic (panel 1) and solid tumour (panel 2). The graphs indicate the densitometric values of corresponding Western blot data. (c) Reduction of secreted serum VEGF levels in DLL‐treated DLA mice in vivo. (d) Gelatin zymography showing reduced gelatinolytic activity in DLL‐treated mice. (e) DLL‐conditioned medium (DLL‐CM) inhibits the migration of A549 cell in vitro. IHV should be replaced with IHC detection of DLL‐induced altered gene expression. (f,i) IHC detection of proangiogenic gene expression in solid tumours in vivo after DLL treatment. The intense brown staining indicates the respective protein expression. Representative graphs demonstrating the gene expression corresponding to the IHC data are given below. (j) Representative counter haematoxylin‐stained section. Data are represented as mean ± standard error of the mean (s.e.m.) of three independent experiments. Statistically significant values are expressed as *P < 0·05 and **P < 0·01. [Colour figure can be viewed at wileyonlinelibrary.com].
Article Snippet: The materials used were lung adenocarcinoma (A549), cervical carcinoma (SiHa, CaSki) and Ehrlich ascites carcinoma (EAC) from the National Centre for Cell Science (NCCS), Pune, India; squamous cell carcinoma (A388) from the National Centre for Biological Science (NCBS), Bengaluru, India and murine Dalton's ascites lymphoma (DLA) cells, a kind gift from Dr Sathees C. Raghavan, Indian Institute of Science (IISc), Bengaluru, India; and human umbilical vascular endothelial cells (HUVEC), endothelial cell growth media (EGM), sodium bicarbonate (NaHCO 3 ), cyanogen bromide (CNBr) activated Sepharose 4B, matrigel [extracellular matrix (ECM) gel], hydron polymer poly‐hydroxyethyl‐methacrylate (poly‐HEMA), Ficoll histopaque, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazoliumbromide (MTT), anti‐vascular endothelial growth factor (VEGF) and anti‐mouse/rabbit immunoglobulin (Ig)G antibodies, protease inhibitor cocktail and Freund's adjuvants were obtained from Sigma‐Aldrich (St Louis, MO, USA), anti‐IL‐2, anti‐hypoxia inducible factor 1α (HIF‐1α); anti‐MMP‐2 and 9 antibodies from Santa Cruz Laboratories (Santa Cruz, CA, USA);
Techniques: Expressing, Western Blot, Zymography, Immunohistochemistry, In Vivo, Activity Assay, Migration, In Vitro, Staining
Journal: Frontiers in Pharmacology
Article Title: Discovery of benzethonium chloride as a potent STAT3 inhibitor for the treatment of HNSCC
doi: 10.3389/fphar.2025.1569570
Figure Lengend Snippet: BZN inhibits STAT3 Y705 phosphorylation and the MAPK pathway. (A, B) Western blot analysis showing downregulation of phosphorylated ERK (p-ERK), p-p38, and p-JNK in CAL27 and FaDu cells treated with BZN (0, 10, and 20 μM). (C, D) Western blot analysis showing decreased phosphorylation of STAT3 at Tyr705 (p-TyrSTAT3), alongside expression changes in STAT1 and p-STAT1 in CAL27 and FaDu cells treated with BZN. (E, F) Expression levels of p53, p-p65, p65, p-IκBα, IκBα, Rel B, p-AKT, and AKT in CAL27 and FaDu cells treated with BZN (0, 10, and 20 μM), analyzed by Western blot.
Article Snippet:
Techniques: Phospho-proteomics, Western Blot, Expressing